Tetrandrine and
fangchinoline were separate in total alkaloids from Stephania tetrandra and the
activities against drug-resistant bacteria in-vitro were investigated.
Preparative medium pressure liquid chromatography and semi-preparative highperformance liquid chromatography were applied to separate and purify
tetrandrine and fangchinoline.
 |
| Tetrandrine and Fangchinoline |
Agar dilution method was used
to test the anti-drug–resistant bacteria activity and determine the minimum
inhibitory concentration (MIC) of MRSA 13366 and ESBLproducing Escherichia coli
13025. Tetrandrine and fangchinoline were identified by TLC and NMR spectra;
The viable count absorbance regression equations were
Y=9.5715x-0.3439(R2=0.9907) for MRSA 13366 and Y=8.6245x-0.1802(R2=0.9972) for
ESBL-producing Escherichia coli 13025; MICs of tetrandrine were 80 μg/ml for
MRSA 13366 and 160 μg/ml for ESBL-producin E. coli 13025; MICs of fangchinoline
were 160 μg/ml for MRSA and 320 μg/ml for ESBL-producing Escherichia coli
13025. Read more>>>>>
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